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PhageUGAL SUMMER SCHOOL

PhageUGALSUMMER SCHOOL

From July 16th to July 19th 2019 the 1st edition of PhageUGAL Summer Schoolwill be held in the Food Science and Engineering Faculty of Dunarea de Jos University Galati Romania. This project will be co-organized with two research scientists from the GutPhageomics group of the APC Microbiome Ireland and one researcher from 4D Pharma PLC, Aberdeen, UK. The workshop series aims to develop skills for potential future scientists towards the application of bacteriophage therapy against emerging antibiotic resistant bacteria. The event includes hands on demonstrations for the isolation and characterization of bacteriophages of problematic bacteria likeÂEscherichia coli, Listeria monocytogenes and Salmonella. Additionally, basic training in molecular techniques (DNA extraction and restriction profiles) and bacteriophage genome annotation will also be taught. Further discussions will cover the topics of phage endolysins and the human gut virome.

Organizing Committee and Lecturers

Andrei Sorin Bolocan is a research scientist at 4D Pharma PLC, Aberdeen, UK, a company focused on developing live biotherapeutic products (LBPs) for the human gut microbiome.LPBs represent a new class of drugs that contain live organisms for the prevention, treatment or cure of diseases as inflammatory bowel syndrome, inflammatory bowel disease, asthma, cancer, Parkinson.

Joan Colom Comas is a postdoctoral researcher at APC Microbiome Ireland - University College Cork, Ireland. He works in a project focused onÂÂisolation and characterization of bacteriophages against specific bacterial targets involved in inflammatory bowel disease (IBD) and exploring the role of bacteriophages in shaping the human gut microbiome during phage therapy.

Colin Buttimer is a postdoctoral researcher at APC Microbiome Ireland - University College Cork who is currently working on isolation and characterization of bacteriophages for application towards the prevention/control of inflammatory bowel disease (IBD).

Leontina Grigore-Gurgu is biologists specialized in molecular biology and bioinformatics. She is lecturer at the Faculty of Food Science and Engineering from the Dunarea de Jos University of Galati, Romania.

Florentina Ionela Bucur is PhD Student at the Faculty of Food Science and Engineering from the Dunarea de Jos University of Galati, Romania, specialization Industrial Engineering, and prepares her thesis on complementary methods to HPP to fight against Listeria monocytogenes.

Anca Ioana Nicolau is professor at the Faculty of Food Science and Engineering from the Dunarea de Jos University of Galati, Romania, where she teaches General Microbiology and Rapid Methods and Automation in Food Microbiology. She coordinates the Food Microbiology Group of the faculty.

Programme

1. Arrival of participants (15 July).

2. Introduction into phage biology; phage isolation, purification, enumeration; phage host range; efficiency of plating (16 – 19 July).

3. Phage DNA extraction methods; DNA restriction profiles (17-18 July).

4. Genome Annotation; In silico prediction of transduction potential (17 July).

5. Gut microbiome challenges: gut bacteriome vs. gut virome (18 July).

7. Hurdles for phage therapy to become a reality; Bacteriophage endolysins (18 July).

6. In silico prediction of prophages in the bacterial genomes; induction of prophages (18 – 19 July).

8. Application of bacteriophages in food industry (19 July).

9. Conclusions. Organisation of the 2nd Edition of PhageUGAL Summer School (19 July).

The exact programme of the event, which will mention starting hours, breaks and working places, will be directly communicated to participants after their registration. The event will combine lectures with laboratory work.

Registration form can be downloaded here.

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